affinity blotting

affinity blotting
Вестерн-блоттинг, белковый б. — техника для выявления белков, аналогичная Саузерн-блоттингу. Белки разделяются при электрофорезе в полиакриламидном геле, переносятся на мембрану и затем метятся с помощью радиоактивной или флуоресцентной метки или с помощью антител (иммунопробы).

Англо-русский толковый словарь генетических терминов. — М.: Изд-во ВНИРО. . 1995.

Игры ⚽ Нужно решить контрольную?

Смотреть что такое "affinity blotting" в других словарях:

  • Far-western blotting — is a molecular biological method which is based on the technique of western blotting. While usual western blotting uses an antibody to detect a protein of interest, far western blotting uses a non antibody protein, which can bind the protein of… …   Wikipedia

  • Immunostaining — Micrograph of an immunostained section of a brain tumour. GFAP immunostain. Immunostaining is a general term in biochemistry that applies to any use of an antibody based method to detect a specific protein in a sample. The term immunostaining was …   Wikipedia

  • Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] …   Wikipedia

  • Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… …   Wikipedia

  • Protein tag — Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes …   Wikipedia

  • Antibody — Immunoglobulin redirects here. For the immunoglobulin family, see Immunoglobulin superfamily. Antibodies redirects here. For the film, see Antibodies (film). Each antibody binds to a specific antigen; an interaction similar to a lock and key. An… …   Wikipedia

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia

  • papermaking — [pā′pər māk΄iŋ] n. the making of paper papermaker n. * * * pa·per·mak·ing (pāʹpər mā kĭng) n. The process or craft of making paper.   paʹper·mak er n. * * * Introduction       formation of a matted or felted sheet, usually of cellulose fibres,… …   Universalium

  • electrochemical reaction — ▪ chemistry Introduction       any process either caused or accompanied by the passage of an electric current and involving in most cases the transfer of electrons between two substances one a solid and the other a liquid.       Under ordinary… …   Universalium


Поделиться ссылкой на выделенное

Прямая ссылка:
Нажмите правой клавишей мыши и выберите «Копировать ссылку»